Overview

  • Product nameAnti-FAK antibody
    See all FAK primary antibodies
  • Description
    Rabbit polyclonal to FAK
  • Specificityab33113 recognises FAK. It does not recognise Pyk2.
  • Tested applicationsSuitable for: WB, ICC, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
    Does not react with: Chicken
  • Immunogen

    pGEX-derived fusion protein, corresponding to amino acids 748/1053 of Human FAK

  • Positive control
    • Ref 52 cells, RIPA lysates from murine 3T3, human A431 RIPA cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab33113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC
IP
  • Application notesICC: Use at a concentration of 10 µg/ml for positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde.
    IP: Use 4 µg to immunoprecipitate FAK from 500µg of murine 3T3 RIPA lysate.
    WB: Use at a concentration of 0.5 - 2 µg/ml to detects FAK in RIPA lysates from murine 3T3 and in human A431 RIPA cell lysate using a chemiluminescence detection system. Predicted molecular weight: 119 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • Tissue specificityExpressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • DomainThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • Post-translational
      modifications
      Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • Cellular localizationCell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Information by UniProt
    • Database links
    • Alternative names
      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    References for Anti-FAK antibody (ab33113)

    ab33113 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab33113.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"