Overview

  • Product nameAnti-FAK antibody
    See all FAK primary antibodies
  • Description
    Rabbit polyclonal to FAK
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from Human FAK around the phosphorylation site of tyrosine 576 (S-T-Y-Y-K).

  • Positive control
    • Human brain tissue and extracts from NIH/3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab61113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 119 kDa (predicted molecular weight: 119 kDa).

Target

  • FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • Tissue specificityExpressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • DomainThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • Post-translational
    modifications
    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • Cellular localizationCell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • Database links
  • Alternative names
    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Anti-FAK antibody images

  • ab61113 at a 1/50 dilution, staining human FAK in brain tissue with (+) or without (-) immunising peptide, using Immunohistochemistry, Paraffin embedded tissue
  • All lanes : Anti-FAK antibody (ab61113) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells
    Lane 2 : Extracts from NIH/3T3 cells with immunising FAK peptide


    Predicted band size : 119 kDa
    Observed band size : 119 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-FAK antibody (ab61113)

This product has been referenced in:
  • Oulidi A  et al. TRPV2 mediates adrenomedullin stimulation of prostate and urothelial cancer cell adhesion, migration and invasion. PLoS One 8:e64885 (2013). WB ; Human . Read more (PubMed: 23741410) »
  • Vandenberghe M  et al. ORAI1 calcium channel orchestrates skin homeostasis. Proc Natl Acad Sci U S A 110:E4839-48 (2013). WB ; Human . Read more (PubMed: 24277812) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Pig Cell lysate - whole cell (Porcine primary aortic endothelial cells)
Gel Running Conditions Reduced Denaturing (12% Bis-Tris gel)
Loading amount 3.8 µg
Specification Porcine primary aortic endothelial cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Jul 03 2015

Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

If a customer would like to test an antibody in an application o...

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Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"