Overview

  • Product nameAnti-FAK antibody [EP1831Y]
    See all FAK primary antibodies
  • Description
    Rabbit monoclonal [EP1831Y] to FAK
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: HeLa cell lysate; IHC-P: human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 119 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • Tissue specificityExpressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • DomainThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • Post-translational
      modifications
      Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • Cellular localizationCell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Information by UniProt
    • Database links
    • Alternative names
      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    Anti-FAK antibody [EP1831Y] images

    • Anti-FAK antibody [EP1831Y] (ab76496) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labeled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 119 kDa
    • Immunohistochemical staining of paraffin-embedded human tonsils using 1/100 ab76496

    References for Anti-FAK antibody [EP1831Y] (ab76496)

    This product has been referenced in:
    • Goodwin JM  et al. An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Mol Cell 55:436-50 (2014). Read more (PubMed: 25042806) »

    See 1 Publication for this product

    Product Wall

    According to our records, ab76496 and ab4803was proving difficult to use inWB and we were in contact in order to help resolve the issue.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"