Anti-FAK (phospho S910) antibody (ab4794)
Key features and details
- Rabbit polyclonal to FAK (phospho S910)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAK (phospho S910) antibody
See all FAK primary antibodies -
Description
Rabbit polyclonal to FAK (phospho S910) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis -
Immunogen
Synthetic peptide corresponding to FAK (phospho S910). The sequence is conserved in human, mouse, rat, chicken and frog.
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General notes
FAK is a non-receptor protein tyrosine kinase discovered as a substrate for Src and it is a key protein in integrin, growth factor and bioactive peptide signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
PBS is without Mg2+ and Ca2+ and BSA is IgG and protease free. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phospho-serine, irrespective of the sequence. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at serine 910. -
Primary antibody notes
FAK is a non-receptor protein tyrosine kinase discovered as a substrate for Src and it is a key protein in integrin, growth factor and bioactive peptide signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 125 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 125 kDa. |
Target
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Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
Tissue specificity
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
Domain
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
Post-translational
modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
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Database links
- Entrez Gene: 396416 Chicken
- Entrez Gene: 5747 Human
- Entrez Gene: 14083 Mouse
- Entrez Gene: 25614 Rat
- Entrez Gene: 399286 Xenopus laevis
- Omim: 600758 Human
- SwissProt: Q00944 Chicken
- SwissProt: Q05397 Human
see all -
Alternative names
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
Images
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Specificity for detecting FAK pS910 phosphorylation using a phospho-specific antibody (PSSA): Cell extracts prepared from mitotic human epithelial carcinoma cells expressing FAK were resolved by SDS-PAGE on a 10% Tris-glycine gel, and transferred to nitrocellulose. Membranes were incubated with 0.5
µ g/mL ab4794, following prior incubation in the presence of the peptide immunogen (lane 1), a generic phosphoserine peptide (lane 2), the non-phosphopeptide corresponding to the FAK phosphopeptide (lane 3), or the absence of the peptide immunogen (lane 4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to the peptide immunogen blocks the antibody signal, thereby demonstrating the specificity of the ab4794 PSSA for the targeted phosphorylation site. Specificity for detecting FAK pS910 phosphorylatio
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab4794 has been referenced in 1 publication.
- Wang YT et al. An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9:5582-97 (2010). WB ; Human . PubMed: 20815410