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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human FAK, phosphorylated at Y397.
(Peptide available as ab40145.)
Our Abpromise guarantee covers the use of ab39967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 119 kDa (predicted molecular weight: 119 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab39967 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.