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Synthetic peptide derived from the region of Focal Adhesion Kinase that contains tyrosine 397. The sequence is conserved in human, mouse, rat, chicken and frog.
Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that was discovered as a substrate for Src, and is a key element of integrin signaling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of Focal Adhesion Kinase. The site binds Src family SH2 domains and the p85 subunit of PI3-Kinase.
Our Abpromise guarantee covers the use of ab4803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 125 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
ab4803 staining FAK (phospho Y397) in Zebrafish fin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/400 in PBS + 0.3% Triton) for 2 hours at 22°C. An undiluted Cy3®-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
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