Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains tyrosine 576. The sequence is conserved in mouse, rat, chicken and frog.
Chicken embryo fibroblasts expressing Focal Adhesion Kinase protein and plated on fibronectin.
Focal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that was discovered as a substrate for Src, and is a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyrosines 576 and 577 are located in the kinase activation loop of Focal Adhesion Kinase and, when phosphorylated by Src, results in maximal activity.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase, (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence and (iii) a phosphopeptide derived from the corresponding region of PYK 2 (a Focal Adhesion Kinase-related protein) to remove antibody that is reactive with PYK 2. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at tyrosine 576.
Primary antibody notesFocal Adhesion Kinase is a 125 kDa non-receptor protein tyrosine kinase that was discovered as a substrate for Src, and is a key element of integrin signalling. Focal Adhesion Kinase plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyrosines 576 and 577 are located in the kinase activation loop of Focal Adhesion Kinase and, when phosphorylated by Src, results in maximal activity.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 125 kDa.
FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Tissue specificityExpressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain.
DomainThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL. The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
Post-translational modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
Protein phosphatase 1 regulatory subunit 71 antibody
Protein tyrosine kinase 2 antibody
Protein-tyrosine kinase 2 antibody
PTK2 protein tyrosine kinase 2 antibody
Anti-FAK (phospho Y576) antibody images
Western blot - FAK (phospho Y576) antibody (ab4815)
Predicted band size : 125 kDa
Peptide Competition: Extracts prepared from chick embryo fibroblasts expressing FAK and plated on fibronectin were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 4% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4815 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the phosphopeptide immunogen (2), the non-phosphopeptide corresponding to the immunogen (3), a phosphopeptide derived from the corresponding region of PYK 2 (4), or, a generic phosphotyrosine containing peptide (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4815 blocks the antibody signal, thereby demonstrating the specificity of the antibody.
References for Anti-FAK (phospho Y576) antibody (ab4815)
This product has been referenced in:
Díaz-Mendoza MJ et al. Interdigital cell death in the embryonic limb is associated with depletion of Reelin in the extracellular matrix. Cell Death Dis4:e800 (2013).
Read more (PubMed: 24030152) »