Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)

Overview

  • Product nameAnti-FAK (phospho Y576) antibody [EP1897Y]
    See all FAK primary antibodies
  • Description
    Rabbit monoclonal [EP1897Y] to FAK (phospho Y576)
  • Specificityab76120 detects FAK phosphorylated at Y576.
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding Tyrosine 576 of human FAK

  • Positive control
    • 3T3 cell lysates treated with pervanadate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/100000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
    • Tissue specificityExpressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • DomainThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
    • Post-translational
      modifications
      Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
    • Cellular localizationCell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
    • Information by UniProt
    • Database links
    • Alternative names
      • FADK 1 antibody
      • FADK antibody
      • FAK related non kinase polypeptide antibody
      • FAK1 antibody
      • FAK1_HUMAN antibody
      • Focal adhesion kinase 1 antibody
      • Focal adhesion Kinase antibody
      • Focal adhesion kinase isoform FAK Del33 antibody
      • Focal adhesion kinase related nonkinase antibody
      • FRNK antibody
      • p125FAK antibody
      • pp125FAK antibody
      • PPP1R71 antibody
      • Protein phosphatase 1 regulatory subunit 71 antibody
      • Protein tyrosine kinase 2 antibody
      • Protein-tyrosine kinase 2 antibody
      • Ptk2 antibody
      • PTK2 protein tyrosine kinase 2 antibody
      see all

    Anti-FAK (phospho Y576) antibody [EP1897Y] images

    • All lanes : Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120) at 1/50000 dilution

      Lane 1 : 3T3 cell lysates untreated
      Lane 2 : 3T3 cell lysates treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 119 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)

    References for Anti-FAK (phospho Y576) antibody [EP1897Y] (ab76120)

    This product has been referenced in:
    • Strell C  et al. Neutrophil granulocytes promote the migratory activity of MDA-MB-468 human breast carcinoma cells via ICAM-1. Exp Cell Res 316:138-48 (2010). Read more (PubMed: 19747913) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    I heard back from the lab explaining that they used permeabilized Jurkat cells, butdo not use mouse samples for flow cytometry. The flow cytometry image is attached.

    Nevertheless, we guarantee the antib...

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    I just emailed the lab regarding the mouse samples tested in flow cytometry.

    The protocol is as follows:

    Flow Cytometry Protocol for Intracellular Staining

    1. Solutions and Reagent...

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    Thank you for contacting us.

    Please find attached the protocol we recomend for ttis antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"