Overview

  • Product nameAnti-FAK (phospho Y861) antibody
    See all FAK primary antibodies
  • Description
    Rabbit polyclonal to FAK (phospho Y861)
  • Specificityab38458 recognises endogenous levels of FAK only when phosphorylated at tyrosine 861.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 861 (HIYpQP).

  • Positive control
    • HepG2, HeLa and 293 cell lysate.
  • General notes


    Tyr861 of Focal Adhesion Kinase is a major Src phosphorylation site. Tyrosine 397 (Tyr397) is the sole autophosphorylation site of FAK. Increased levels of phosphorylated Tyr397 (P-Tyr397) thus correlate well with FAK activation. Recent studies have shown that FAK phosphorylation at Tyr861 promotes both FAK binding to alpha(v)beta5 integrin and Tyr397 phosphorylation.

Properties

Applications

Our Abpromise guarantee covers the use of ab38458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 119 kDa.
ELISA 1/20000.

Target

  • FunctionNon-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
  • Tissue specificityExpressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • DomainThe first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
  • Post-translational
    modifications
    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.
  • Cellular localizationCell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt
  • Database links
  • Alternative names
    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

Anti-FAK (phospho Y861) antibody images

  • All lanes : Anti-FAK (phospho Y861) antibody (ab38458) at 1/500 dilution

    Lane 1 : HepG2 cells lysate pretreated with phosphopeptide (negative control).
    Lane 2 : Untreated HepG2 cell lysate
    Lane 3 : Untreated HeLa cell lysate
    Lane 4 : EGF treated 293 cell lysate.

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 119 kDa
    Observed band size : 119 kDa

References for Anti-FAK (phospho Y861) antibody (ab38458)

ab38458 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab38458.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"