The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 122 kDa.
Use at an assay dependent concentration.
FunctionMay participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.
Tissue specificityUbiquitously expressed. Highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa (at protein level).
Sequence similaritiesBelongs to the constitutive coactivator of PPAR-gamma family.
Post-translational modificationsArg-982 is dimethylated, probably to asymmetric dimethylarginine. Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure.
Cellular localizationCytoplasm. Cell membrane. Translocates to plasma membrane upon ultraviolet exposure.