May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.
Ubiquitously expressed. Highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa (at protein level).
Belongs to the constitutive coactivator of PPAR-gamma family.
Arg-982 is dimethylated, probably to asymmetric dimethylarginine. Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure.
Cytoplasm. Cell membrane. Translocates to plasma membrane upon ultraviolet exposure.
All lanes : Anti-FAM120A antibody (ab156695) at 1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg Lane 2 : Jurkat whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Detection of FAM120A by Western blot of immunoprecipitate.
Immunoprecipitation of FAM120A (6 µg of antibody per mg of Jurkat whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: alternative rabbit anti-FAM120A antibody
Lane 2: ab156695
Lane 3: Control IgG
For the subsequent Western blot of immunoprecipitates, ab156695 was used at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.