Overview

  • Product name
  • Description
    Rabbit polyclonal to FAM120A
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Hamster, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1068-1118 of Human FAM120A (NP_055427.2).

  • Positive control
    • 293T, Jurkat, NIH 3T3 and HeLa whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156695 was affinity purified using an epitope specific to FAM120A immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 121 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.
  • Tissue specificity
    Ubiquitously expressed. Highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa (at protein level).
  • Sequence similarities
    Belongs to the constitutive coactivator of PPAR-gamma family.
  • Post-translational
    modifications
    Arg-982 is dimethylated, probably to asymmetric dimethylarginine.
    Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocates to plasma membrane upon ultraviolet exposure.
  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf10 antibody
    • Chromosome 9 open reading frame 10 antibody
    • Constitutive coactivator of PPAR gamma like protein 1 antibody
    • Constitutive coactivator of PPAR-gamma-like protein 1 antibody
    • DNA polymerase transactivated protein 5 antibody
    • DNA polymerase transactivated protein 1 antibody
    • DNAPTP1 antibody
    • DNAPTP5 antibody
    • F120A_HUMAN antibody
    • FAM120A antibody
    • Family with sequence similarity 120A antibody
    • Hypothetical protein LOC23196 antibody
    • KIAA0183 antibody
    • LOC23196 antibody
    • MGC111527 antibody
    • MGC133257 antibody
    • OSSA antibody
    • Oxidative stress-associated Src activator antibody
    • Protein FAM120A antibody
    see all

Images

  • All lanes : Anti-FAM120A antibody (ab156695) at 1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg


    Predicted band size : 121 kDa
  • Detection of FAM120A by Western blot of immunoprecipitate.
    Immunoprecipitation of FAM120A (6 µg of antibody per mg of Jurkat whole cell lysate; 1 mg/IP; 20% of IP loaded/lane), using the following:
    Lane 1: alternative rabbit anti-FAM120A antibody
    Lane 2: ab156695
    Lane 3: Control IgG
    For the subsequent Western blot of immunoprecipitates, ab156695 was used at 1 µg/ml.
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab156695 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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