Anti-FAM134C antibody - C-terminal (ab202125)
Key features and details
- Rabbit polyclonal to FAM134C - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAM134C antibody - C-terminal
See all FAM134C primary antibodies -
Description
Rabbit polyclonal to FAM134C - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide within Human FAM134C aa 416-466 (C terminal). The exact sequence is proprietary. NP_835227.1
Database link: Q86VR2 -
Positive control
- HeLa, 293T, and Jurkat whole cell lysate (ab7899).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 51 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Sequence similarities
Belongs to the FAM134 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 741874 Chimpanzee
- Entrez Gene: 789163 Cow
- Entrez Gene: 490964 Dog
- Entrez Gene: 100065999 Horse
- Entrez Gene: 162427 Human
- Entrez Gene: 67998 Mouse
- Entrez Gene: 100455363 Orangutan
- Entrez Gene: 100519106 Pig
see all -
Alternative names
- DKFZp686B1036 antibody
- F134C_HUMAN antibody
- fam134c antibody
see all
Images
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All lanes : Anti-FAM134C antibody - C-terminal (ab202125) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 30 seconds -
Lane 1: Detection of FAM134C in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 0.5 or 1.0 mg per IP reaction, 20% of IP loaded) using ab202125 at 6 µg per reaction, and at 1 µg/ml for subsequent Western blot detection.
Lane 2: controle IgG
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab202125 has not yet been referenced specifically in any publications.