Overview

  • Product name
  • Description
    Rabbit polyclonal to FAM135A
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 1148-1230 (CTSGCLSFPS APRESPCNVK YSSKSKFDAI TKQPSSTSYN FTSSISWYES SPKPQIQAFL QAKEELKLLK LPGFMYSEVP LLA) of Human FAM135A.

  • Positive control
    • Human kidney tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the FAM135 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4921533L14Rik antibody
    • AI256732 antibody
    • AI835491 antibody
    • F135A_HUMAN antibody
    • Fam135a antibody
    • Family with sequence similarity 135, member A antibody
    • KIAA1411 antibody
    • Protein FAM135A antibody
    • RGD1304927 antibody
    • RP1-198I9.1 antibody
    • Uncharacterized protein LOC367235 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122190 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122190 at 1/15 dilution staining FAM135A in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.

References

ab122190 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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