Overview

  • Product nameAnti-FAM151B antibody
  • Description
    Rabbit polyclonal to FAM151B
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: MAASAGGPGS WSENILEYFL RNSQITAEDG AEITWYHAAN HKAQTNEALK STAHMIEADV LLPSDGSEHS QPIMAHPPET NSDNTLQEWL TEVMK, corresponding to N terminal amino acids 1-95 of Human FAM151B.

  • Positive control
    • Human colon tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-FAM151B antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab122863 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
  • ab122863 at 1/50 dilution staining FAM151B in paraffin-embedded Human colon tissue by Immunohistochemistry.

References for Anti-FAM151B antibody (ab122863)

ab122863 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122863.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"