Overview

  • Product nameAnti-FAM154B antibody
    See all FAM154B primary antibodies
  • Description
    Rabbit polyclonal to FAM154B
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: QGLIGETAKL CRPVHTRVTQ NALFEGSTEF RESFQPWEIP PPEVKKVPEY VPPTGSMLLN STSHLDYVPY QANHVVPIR, corresponding to internal sequence amino acids 189-267 of Human FAM154B. (Q658L1).

  • Positive control
    • Human colon tissue. RT-4 and U-251 MG cell lysates. Human plasma lysate. Human liver and tonsil tissue lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-FAM154B antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122655 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122655 at 1/50 dilution staining FAM154B in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-FAM154B antibody (ab122655) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique

References for Anti-FAM154B antibody (ab122655)

ab122655 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"