Overview

  • Product nameAnti-FAM172A antibody
  • Description
    Rabbit polyclonal to FAM172A
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen, corresponding to amino acids 19-135 of Human FAM172A (Uniprot: Q8WUF8)

  • Positive control
    • Human Cerebellum tissue, RT-4 lysate, U-251 MG lysate, Liver lysate and Tonsil lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-FAM172A antibody images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121364 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121364, at 1/200 dilution, staining FAM172A in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
  • All lanes : Anti-FAM172A antibody (ab121364) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Liver lysate
    Lane 5 : Tonsil lysate

References for Anti-FAM172A antibody (ab121364)

ab121364 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"