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Rabbit polyclonal to FAM172A
Mouse, Rat, Human
antigen, corresponding to amino acids 19-135 of Human FAM172A (Uniprot: Q8WUF8)
Human Cerebellum tissue, RT-4 lysate, U-251 MG lysate, Liver lysate and Tonsil lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Belongs to the UPF0528 family.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-FAM172A antibody (ab121364)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121364 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM172A antibody (ab121364)
ab121364, at 1/200 dilution, staining FAM172A in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
Western blot - Anti-FAM172A antibody (ab121364)
All lanes : Anti-FAM172A antibody (ab121364) at 1/250 dilution Lane 1 : RT-4 lysate Lane 2 : U-251 MG lysate Lane 3 : Human Plasma lysate Lane 4 : Liver lysate Lane 5 : Tonsil lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"