Overview

  • Product name
    Anti-FAM174A antibody
  • Description
    Rabbit polyclonal to FAM174A
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: RRNRKTRRYG VLDTNIENME LTPLEQDDED DDNTLFDANH PRR, corresponding to C terminal amino acids 148-190 of the Human FAM174A protein.

  • Positive control
    • Human hippocampus tissue; Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Belongs to the FAM174 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • F174A_HUMAN antibody
    • FAM174A antibody
    • HCV NS5A-transactivated protein 6 antibody
    • Hepatitis C virus NS5A-transactivated protein 6 antibody
    • Membrane protein FAM174A antibody
    • NS5ATP6 antibody
    • RGD1309973 antibody
    • TMEM157 antibody
    • Transmembrane protein 157 antibody
    • UNQ1912/PRO4371 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus. Recommended concentration of ab121607 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121607 at 1/120 dilution staining FAM174A in Paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
    Note: strong cytoplasmic positivity with a granular pattern in neuronal cells.
  • All lanes : Anti-FAM174A antibody (ab121607) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate [Co-expressed with a C-terminal proprietary tag (~3.1 kDa)] in mammalian HEK293T cells.

    Developed using the ECL technique

References

ab121607 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121607.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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