Overview

  • Product name
  • Description
    Mouse polyclonal to FAM175B
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein, corresponding to amino acids 105-415a.a. of Human FAM175B

  • Positive control
    • FAM175B transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 47 kDa).

Target

  • Relevance
    FAM175B (ABRO1) is a 415 amino acid protein, sharing 39% identity to ABRA1 in the N terminal two thirds of the protein (amino acids 1 to 260), including a coiled-coil domain and a region called the ABR domain. However, ABRO1 lacks the phosphorylated ser-X-X-phe motif of ABRA1 and, unlike ABRA1, does not bind to BRCA1.
  • Database links
  • Alternative names
    • AA589499 antibody
    • Abraxas brother 1 antibody
    • ABRO 1 antibody
    • ABRO antibody
    • ABRO1 antibody
    • AI853413 antibody
    • C430003P19Rik antibody
    • Em:AC068896.4 antibody
    • Family with sequence similarity 175, member B antibody
    • FLJ22338 antibody
    • hypothetical protein LOC23172 antibody
    • KIAA0157 antibody
    • OTTHUMP00000020697 antibody
    • RGD1308918 antibody
    see all

Images

  • All lanes : Anti-FAM175B antibody (ab68801) at 1/500 dilution

    Lane 1 : FAM175B (1-311 a.a.: 34.7kDa) transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L) at 1/5000 dilution

    Predicted band size: 47 kDa
    Observed band size: 43 kDa (why is the actual band size different from the predicted?)

References

ab68801 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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