Overview

  • Product name
  • Description
    Rabbit polyclonal to FAM175B
  • Specificity
    The antibody recognises a region between residue 350 and 390 of human FAM175B using the numbering given in entry NP_115558.3 (GeneID 23172).
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 350 and 390 of human FAM175B (NP_115558.3)

  • Positive control
    • Whole HeLa cell lysate. Whole 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibody was affinity purified using an epitope specific to FAM175B immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    FAM175B (ABRO1) is a 415 amino acid protein, sharing 39% identity to ABRA1 in the N terminal two thirds of the protein (amino acids 1 to 260), including a coiled-coil domain and a region called the ABR domain. However, ABRO1 lacks the phosphorylated ser-X-X-phe motif of ABRA1 and, unlike ABRA1, does not bind to BRCA1.
  • Database links
  • Alternative names
    • AA589499 antibody
    • Abraxas brother 1 antibody
    • ABRO 1 antibody
    • ABRO antibody
    • ABRO1 antibody
    • AI853413 antibody
    • C430003P19Rik antibody
    • Em:AC068896.4 antibody
    • Family with sequence similarity 175, member B antibody
    • FLJ22338 antibody
    • hypothetical protein LOC23172 antibody
    • KIAA0157 antibody
    • OTTHUMP00000020697 antibody
    • RGD1308918 antibody
    see all

Images

  • ab74333 at 1 ug/ml (used for IP at 3 ug/lysate).
    Lane 1: Whole HeLa cell lysate at 1 mg for IP (20% of IP loaded).
    Lane 2: control IgG.
    Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab74333 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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