Overview

  • Product name
  • Description
    Rabbit polyclonal to FAM175B
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 366-415 (GDSGEDSDDS DYENLIDPTE PSNSEYSHSK DSRPMAHPDE DPRNTQTSQI) of Human FAM175B (NP_115558).

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    FAM175B (ABRO1) is a 415 amino acid protein, sharing 39% identity to ABRA1 in the N terminal two thirds of the protein (amino acids 1 to 260), including a coiled-coil domain and a region called the ABR domain. However, ABRO1 lacks the phosphorylated ser-X-X-phe motif of ABRA1 and, unlike ABRA1, does not bind to BRCA1.
  • Database links
  • Alternative names
    • AA589499 antibody
    • Abraxas brother 1 antibody
    • ABRO 1 antibody
    • ABRO antibody
    • ABRO1 antibody
    • AI853413 antibody
    • C430003P19Rik antibody
    • Em:AC068896.4 antibody
    • Family with sequence similarity 175, member B antibody
    • FLJ22338 antibody
    • hypothetical protein LOC23172 antibody
    • KIAA0157 antibody
    • OTTHUMP00000020697 antibody
    • RGD1308918 antibody
    see all

Images

  • Anti-FAM175B antibody (ab83860) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References

ab83860 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83860.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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