Overview

  • Product nameAnti-FAM179A antibody
  • Description
    Rabbit polyclonal to FAM179A
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    internal sequence amino acids 742-834 (GLSCNGPRLV GLRSTLQGRG EMVEQLRELT RLLEAKDFRS RMEGVGQLLE LCKAKTELVT AHLVQVFDAF TPRLQDSNKK VNQWALESFA KMI) of the Human FAM179A protein (Q6ZUX3).

  • Positive control
    • IHC-P: Human colon tissue WB: RT4, U251 MG, Human Liver and Tonsil cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-FAM179A antibody images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus and nucleoli. Recommended concentration of ab122395 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-FAM179A antibody (ab122395) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human Plasma cell lysate
    Lane 4 : Liver cell lysate
    Lane 5 : Tonsil cell lysate
  • ab122395, at 1/50, staining FAM179A in paraffin embedded Human colon tissue by immunohistochemistry.

References for Anti-FAM179A antibody (ab122395)

ab122395 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"