Overview

  • Product name
    Anti-FAM184A antibody
  • Description
    Rabbit polyclonal to FAM184A
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 262-365 of Human FAM184A.

  • Positive control
    • Human heart muscle tissue; HEK293T cell FAM184A over-expression lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-FAM184A antibody images

  • ab122215, at 1/300 dilution, staining FAM184A in paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
  • All lanes : Anti-FAM184A antibody (ab122215) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : FAM184A over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique
  • Immunohistochemical analysis of Human umbilical vein endothelial cells, staining FAM184A with ab122215.

    Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 2% BSA for 45 minutes at 25°. Samples were incubated with primary antibody (5 µg/ml in diluent) for 1 hour at 25°C. A Cy3®-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-FAM184A antibody (ab122215)

ab122215 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human umbilical vein endothelial cells)
Specification
human umbilical vein endothelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - triton X
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 01 2012

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa and RPE-1)
Loading amount
50 µg
Specification
HeLa and RPE-1
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris (MES Buffer))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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