Overview

  • Product name
    Anti-FAM184A antibody
  • Description
    Rabbit polyclonal to FAM184A
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 262-365 of Human FAM184A.

  • Positive control
    • Human heart muscle tissue; HEK293T cell FAM184A over-expression lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • ab122215, at 1/300 dilution, staining FAM184A in paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
  • All lanes : Anti-FAM184A antibody (ab122215) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : FAM184A over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique
  • Immunohistochemical analysis of Human umbilical vein endothelial cells, staining FAM184A with ab122215.

    Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 2% BSA for 45 minutes at 25°. Samples were incubated with primary antibody (5 µg/ml in diluent) for 1 hour at 25°C. A Cy3®-conjugated donkey anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

References

ab122215 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human umbilical vein endothelial cells)
Specification
human umbilical vein endothelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - triton X
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 01 2012

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa and RPE-1)
Loading amount
50 µg
Specification
HeLa and RPE-1
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris (MES Buffer))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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