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Rabbit polyclonal to FAM186A
antigen sequence: ETVTKILRKY KDTKKEEQVG EKPIKQKKVV SFMPGLHFQK SPISAKSESS TLLSYESTDP VINNLIQMIL AEIESERDIP TVSTVQKDHK EK, corresponding to amino acids 726-818 of Human FAM186A. (A6NE01).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/1000 - 1/2500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the FAM186 family.
Information by UniProt
- F186A_HUMAN antibody
- FAM186A antibody
- LOC121006 antibody
- Protein FAM186A antibody
Immunocytochemistry/ Immunofluorescence - Anti-FAM186A antibody (ab126525)
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab126525 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-FAM186A antibody (ab126525)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM186A antibody (ab126525)
ab126525, at 1/50 dilution, staining FAM186A in paraffin-embedded Human stomach tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"