Overview

  • Product name
    Anti-FAM186A antibody
  • Description
    Rabbit polyclonal to FAM186A
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ETVTKILRKY KDTKKEEQVG EKPIKQKKVV SFMPGLHFQK SPISAKSESS TLLSYESTDP VINNLIQMIL AEIESERDIP TVSTVQKDHK EK, corresponding to amino acids 726-818 of Human FAM186A. (A6NE01).

  • Positive control
    • Human stomach tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/1000 - 1/2500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab126525 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: RT-4
    Lane 3: U-251 MG
    Lane 4: Human Plasma
    Lane 5: Liver
    Lane 6: Tonsil
  • ab126525, at 1/50 dilution, staining FAM186A in paraffin-embedded Human stomach tissue by Immunohistochemistry.

References

ab126525 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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