Overview

  • Product nameAnti-FAM199X antibody
  • Description
    Rabbit polyclonal to FAM199X
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 181-309 of Human FAM199X.

  • Positive control
    • Human cerebral cortex tissue; RT4 lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

Anti-FAM199X antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab121167 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121167 at 1/10 dilution staining FAM199X in Paraffin-emebedded Human cerebral cortex tissue by Immunohistochemistry.

    Note: strong nuclear and moderate cytoplasmic positivity in neuronal cells.
  • All lanes : Anti-FAM199X antibody (ab121167) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U251 MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References for Anti-FAM199X antibody (ab121167)

ab121167 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"