Overview

  • Product name
    Anti-FAM20B antibody
  • Description
    Rabbit polyclonal to FAM20B
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 258-402 of Human FAM20B.

  • Positive control
    • Human duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function
    Responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature GAG chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-o-phosphorylation may influence the catalytic activity of B3GAT3 (GlcAT-I) which completes the precursor tetrasaccharide of GAG-protein linkage regions on which the repeating disaccharide region is synthesized.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the FAM20 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fam20b antibody
    • Family with sequence similarity 20 member B antibody
    • Glycosaminoglycan xylosylkinase antibody
    • Gxk1 antibody
    • Protein FAM20B antibody
    • XYLK_HUMAN antibody
    • Xylose kinase antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling FAM20B with ab121261 shows positivity in the Golgi apparatus & nucleus but excluded from the nucleoli.

  • ab121261, at 1/200 dilution, staining FAM20B in paraffin-embedded Human duodenum tissue by Immunohistochemistry, showing granular cytoplasmic positivity in glandular cells.

References

ab121261 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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