Overview

  • Product nameAnti-FAM20C antibody
    See all FAM20C primary antibodies
  • Description
    Rabbit polyclonal to FAM20C
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide between amino acids 443-471 from the C terminal region of Human FAM20C (NP_064608.2).

  • Positive control
    • HepG2 cell lysate; Human breast carcinoma tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab107079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 64 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionCalcium-binding protein which may play a role in dentin mineralization.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in FAM20C are the cause of Raine syndrome (RNS) [MIM:259775]. RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly.
  • Sequence similaritiesBelongs to the FAM20 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C76981 antibody
    • Dentin matrix protein 4 antibody
    • DKFZp547C074 antibody
    • DMP-4 antibody
    • DMP4 antibody
    • DMP4_HUMAN antibody
    • Extracellular serine/threonine protein kinase Fam20C antibody
    • Fam20c antibody
    • Family with sequence similarity 20 member C antibody
    • GEF CK antibody
    • Golgi enriched fraction casein kinase antibody
    • Protein FAM20C antibody
    • RNS antibody
    see all

Anti-FAM20C antibody images

  • ICC/IF image of ab107079 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107079 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-FAM20C antibody (ab107079) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 64 kDa
  • ab107079, at a 1/50 dilution, staining FAM20C in formalin fixed, paraffin embedded Human breast carcinoma by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-FAM20C antibody (ab107079)

ab107079 has not yet been referenced specifically in any publications.

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