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Rabbit polyclonal to FAM20C
Predicted to work with:
Mouse, Rat, Monkey
Recombinant fragment corresponding to a region within amino acids 233-512 of Human FAM20C (UniProt ID: Q8IXL6).
Molt4 whole cell lysate; Hep3B cells; Human MDA-MB468 xenograft.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 66 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
1/100 - 1/1000.
Calcium-binding protein which may play a role in dentin mineralization.
Involvement in disease
Defects in FAM20C are the cause of Raine syndrome (RNS) [MIM:259775]. RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly.
Belongs to the FAM20 family.
Information by UniProt
Dentin matrix protein 4 antibody
Western blot - Anti-FAM20C antibody (ab154740)
Anti-FAM20C antibody (ab154740) at 1/500 dilution + Molt4 whole cell lysate at 30 µg
Predicted band size: 66 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-FAM20C antibody (ab154740)
Immunofluorescent analysis of methanol-fixed Hep3B cells labeling FAM20C with ab154740 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM20C antibody (ab154740)
Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft labeling FAM20C with ab154740 at 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"