Anti-FAM32A/OTAG12 antibody (ab185298)
Key features and details
- Rabbit polyclonal to FAM32A/OTAG12
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FAM32A/OTAG12 antibody -
Description
Rabbit polyclonal to FAM32A/OTAG12 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human FAM32A/OTAG12 aa 50 to the C-terminus.
Database link: Q9Y421 -
Positive control
- WB: PC-3 cell lysate; IHC-P: Human small intestine, skin, and pancreas tissues; ICC: A-431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
FAM32A belongs to the FAM32 family. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 26017 Human
- Entrez Gene: 67922 Mouse
- Entrez Gene: 498600 Rat
- SwissProt: Q9Y421 Human
- SwissProt: Q9CR80 Mouse
- SwissProt: B2RYG1 Rat
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Alternative names
- CGI 144 antibody
- DKFZP586O0120 antibody
- Family with sequence similarity 32 member A antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling FAM32A/OTAG12 with ab185298 at 1/200 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed, Triton X-100-permeabilized A431 cells labeling FAM32A/OTAG12 with ab185298 at 4 µg/ml.
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Anti-FAM32A/OTAG12 antibody (ab185298) at 0.4 µg/ml + PC-3 (human prostate adenocarcinoma cell line) cell lysate
Predicted band size: 13 kDa -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling FAM32A/OTAG12 with ab185298 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded human skin tissue labelling FAM32A/OTAG12 with ab185298 at 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab185298 has not yet been referenced specifically in any publications.