Anti-FAM38A antibody (ab82336)

Overview

  • Product nameAnti-FAM38A antibody
    See all FAM38A primary antibodies
  • Description
    Rabbit polyclonal to FAM38A
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human FAM38A.

    (Peptide available as ab95859.)

  • Positive control
    • This antibody gave a positive signal in the following cell lysates: HeLa; HepG2. ICC/IF: PC12 cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab82336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 286 kDa (predicted molecular weight: 286 kDa).

Target

  • FunctionComponent of mechanosensitive channel required for the mechanosensitive currents. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling.
  • Tissue specificityExpressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes.
  • Sequence similaritiesBelongs to the PIEZO family.
  • Cellular localizationEndoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHS antibody
    • Fam38a antibody
    • Family with sequence similarity 38 member A antibody
    • KIAA0233 antibody
    • Membrane protein induced by beta-amyloid treatment antibody
    • Mib antibody
    • PIEZ1_HUMAN antibody
    • Piezo-type mechanosensitive ion channel component 1 antibody
    • PIEZO1 antibody
    • Protein FAM38A antibody
    • Protein FAM38B antibody
    • Protein PIEZO1 antibody
    see all

Anti-FAM38A antibody images

  • All lanes : Anti-FAM38A antibody (ab82336) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (unheated)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (unheated)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 286 kDa
    Observed band size : 286 kDa
    Additional bands at : 56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab82336 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • ICC/IF image of ab82336 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82336, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 5µg/ml.

References for Anti-FAM38A antibody (ab82336)

ab82336 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (vascular smooth muscle cell)
Gel Running Conditions Reduced Denaturing
Loading amount 250 µg
Treatment control,negative control,SiRNA for 48hrs
Specification vascular smooth muscle cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 22 2015

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-20 %)
Sample Human Cell lysate - whole cell (Keratinocyte from epidermis)
Specification Keratinocyte from epidermis
Blocking step Immunoblock as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
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Submitted Oct 06 2014

Thank you for contacting us.

The band observed is indeed ˜280 kDa with HepG2 whole cell lysates. http://www.uniprot.org/uniprot/Q92508

There are 4 isoforms for Piezo 2 protein. http://www.uniprot.org/uniprot/Q9H5I5

The Piezo...

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Thank you very much for your interest.

Our Abreview Testing Discount Program exists for those times when a product has not, to our knowledge, been tested in an application or species but there is not reseaon to believe that it would not wo...

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Thanks for the additional details.

Since you're using frozen sections, I'd suggest using ab128245 as its already been tested in IHC-Fr on human samples. Although it has not been tested on mouse or rat samples that we know of, based on the sim...

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Thank you for your enquiry.

I would be happy to help you choose the most appropriate antibody for your IHC experiment. I just have a couple additional questions about your experiment:

1. Will you be using frozen sections or paraffin...

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