Overview

  • Product name
  • Description
    Rabbit polyclonal to FAM38B
  • Specificity
    Specific to isoforms 1, 2, 3 and 4.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide: AGNSTESSKT PVTIEKIYPY YVKAPSDSNS KPIKQLLSEN NFMDITIILS, corresponding to amino acids 2503-2552 of Human FAM38B

  • Positive control
    • 721_B cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 318 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

Anti-FAM38B antibody images

  • Anti-FAM38B antibody (ab81327) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 318 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Isoform 3 has predicted MW 81 kDa.

    Gel concentration: 12%

References for Anti-FAM38B antibody (ab81327)

ab81327 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab81327.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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