Overview

  • Product nameAnti-FAM38B antibody
    See all FAM38B primary antibodies
  • Description
    Rabbit polyclonal to FAM38B
  • SpecificitySpecific to isoforms 1, 2, 3 and 4.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide: AGNSTESSKT PVTIEKIYPY YVKAPSDSNS KPIKQLLSEN NFMDITIILS, corresponding to amino acids 2503-2552 of Human FAM38B

  • Positive control
    • 721_B cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 318 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

Anti-FAM38B antibody images

  • Anti-FAM38B antibody (ab81327) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 318 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Isoform 3 has predicted MW 81 kDa.

    Gel concentration: 12%

References for Anti-FAM38B antibody (ab81327)

ab81327 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab81327.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"