Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 26 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionInduces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Present in insulin secretory granules and likely cosecreted with insulin.
  • Tissue specificityHighly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis.
  • Sequence similaritiesBelongs to the FAM3 family.
  • Post-translational
    modifications
    2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46.
  • Cellular localizationSecreted. Localized in discrete vesicular and perinuclear structure.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-21 antibody
    • C21orf11 antibody
    • C21orf76 antibody
    • Cytokine like protein 2 21 antibody
    • Cytokine-like protein 2-21 antibody
    • Fam3b antibody
    • FAM3B_HUMAN antibody
    • Family with sequence similarity 3 member B antibody
    • ORF9 antibody
    • Pancreatic-derived factor antibody
    • PANDER antibody
    • PRED44 antibody
    • Protein FAM3B antibody
    see all

Anti-FAM3B antibody images

  • Anti-FAM3B antibody (ab88142) at 1 µg/ml + immunogen (recombinant full length protein with proprietary tag of 26kDa) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 26 kDa
  • Anti-FAM3B antibody (ab88142) at 5 µg/ml + Human kidney tissue lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 26 kDa
  • All lanes : Anti-FAM3B antibody (ab88142) at 5 µg/ml

    Lane 1 : FAM3B-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Goat anti-Mouse IgG at 1/2500 dilution
    Lane 2 : Goat anti-Mouse IgG at 1/2500 dilution


    Predicted band size : 26 kDa

References for Anti-FAM3B antibody (ab88142)

ab88142 has not yet been referenced specifically in any publications.

Product Wall

Thank you for sending your customer's protocol.

It is mostly correct, but for 293T cells, the antibody needs to be used at a concentration of at least 5ug/ml (1/200) in order to develop even a weak signal. The datasheet shows an image of a 2...

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