Overview

  • Product name
  • Description
    Rabbit polyclonal to FAM3C
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Chicken, Cow, Dog, Human, Monkey
    Predicted to work with: Mouse, Rat, Chimpanzee, Pufferfish, Opossum
  • Immunogen

    Synthetic peptide corresponding to a portion of amino acids 40-80 of Human FAM3C

  • Positive control
    • Human, mouse and rat brain lysates. Human brain and skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab72182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • All lanes : Anti-FAM3C antibody (ab72182) at 0.5 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate with immunising peptide
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate


    Predicted band size : 25 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab72182 at 5µg/ml staining FAM3C in normal human brain by Immunohistochemistry using paraffin-embedded tissue. (Control rabbit Ig used for figure B).
  • ab72182 at 10µg/ml staining FAM3C in human skin by Immunohistochemistry using paraffin-embedded tissue.
  • ICC/IF image of ab72182 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72182, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Noguchi K  et al. Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines. PLoS One 12:e0177830 (2017). Read more (PubMed: 28545079) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Prostate)
Specification
Prostate
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate)
Specification
prostate
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

Submitted Dec 08 2011

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse mammary cell)
Loading amount
50 µg
Specification
Mouse mammary cell
Treatment
5ng/ml TGF beta for different time periods (hrs)
Gel Running Conditions
Reduced Denaturing (10.0% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 31 2011

Application
Immunocytochemistry
Sample
Human Cell (Prostate)
Specification
Prostate
Fixative
Methanol
Permeabilization
Yes - Triton X-100
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

Submitted May 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate)
Loading amount
25 µg
Specification
Prostate
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

Submitted Apr 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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