Overview

  • Product nameAnti-FAM48A antibody
    See all FAM48A primary antibodies
  • Description
    Rabbit polyclonal to FAM48A
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 98-191 (DSETIRLPYE EGELLEYLDA EELPPILVDL LEKSQVNIFH CGCVIAEIRD YRQSSNMKSP GYQSRHILLR PTMQTLICDV HSITSDNHKW TQED) of Human FAM48A.

  • Positive control
    • Human pancreas tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRequired for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down-regulation of E-cadherin during gastrulation by regulating E-cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by Fgf signaling and Snail (By similarity). Required for starvation-induced ATG9A trafficking during autophagy.
  • Tissue specificityHighly expressed in testis, moderately in brain and pituitary gland. Expressed in several fetal tissues, including lung, brain, thymus and kidney. Expression is down-regulated in malignant prostate tissues.
  • Sequence similaritiesBelongs to the FAM48 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA667204 antibody
    • AI450544 antibody
    • bA421P11.4 antibody
    • C13 antibody
    • C13orf19 antibody
    • D3Ertd300e antibody
    • FA48A_HUMAN antibody
    • FAM48A antibody
    • FP757 antibody
    • p38-interacting protein antibody
    • p38IP antibody
    • Protein FAM48A antibody
    • RGD1307812 antibody
    • SPT20 antibody
    see all

Anti-FAM48A antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122517 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-FAM48A antibody (ab122517)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing FAM48A with a C-terminal myc-DDK tag (~3.1kDa)
  • ab122517 at 1/400 dilution staining FAM48A in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.

References for Anti-FAM48A antibody (ab122517)

ab122517 has not yet been referenced specifically in any publications.

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