Anti-FAM49B antibody (ab121299)
Key features and details
- Rabbit polyclonal to FAM49B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FAM49B antibody -
Description
Rabbit polyclonal to FAM49B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 2-134 of Human FAM49B (Q9NUQ9).
-
Positive control
- RT4, U-251 MG and Human Tonsil lysate; Human kidney tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
Notes |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
-
Sequence similarities
Belongs to the FAM49 family. - Information by UniProt
-
Database links
- Entrez Gene: 51571 Human
- SwissProt: Q9NUQ9 Human
- Unigene: 126941 Human
-
Alternative names
- BM 009 antibody
- FA49B_HUMAN antibody
- Fam49b antibody
see all
Images
-
All lanes : Anti-FAM49B antibody (ab121299) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : Human Plasma lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab121299 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
ab121299 at 1/100 dilution staining FAM49B in Paraffin-emebedded Human kidney tissue by Immunohistochemistry.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab121299 has been referenced in 1 publication.
- Zhang Y et al. TASP1 Promotes Gallbladder Cancer Cell Proliferation and Metastasis by Up-regulating FAM49B via PI3K/AKT Pathway. Int J Biol Sci 16:739-751 (2020). PubMed: 32071545