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Rabbit polyclonal to FAM62A
Synthetic peptide corresponding to a region within amino acids 1054-1104 of Human FAM62A (NP_056107.1).
HeLa, 293T and Jurkat whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab118805 was affinity purified using an epitope specific to FAM62A immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 123 kDa.
Use at 2-10 µg/mg of lysate.
May play a role as calcium-regulated intrinsic membrane protein.
Belongs to the extended synaptotagmin family.
Contains 5 C2 domains.
Membrane. Localizes to intracellular membranes.
Information by UniProt
E Syt1 antibody
Western blot - Anti-FAM62A antibody (ab118805)
All lanes : Anti-FAM62A antibody (ab118805) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 123 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-FAM62A antibody (ab118805)
Detection of FAM62A in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118805 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
has not yet been referenced specifically in any publications.
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