For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- PurityImmunogen affinity purified
- Research areas
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6.0
- Sequence similaritiesBelongs to the FAM65 family.
- Cellular localizationCytoplasm. Localizes to the podocyte major processes and cell body.
Information by UniProt
- FA65A_HUMAN antibody
- Fam65a antibody
- Protein FAM65A antibody
Anti-FAM65A antibody images
Immunocytochemistry/ Immunofluorescence - Anti-FAM65A antibody (ab121240)
Immunofluorescent staining of Human cell line U-251MG shows positivity in golgi apparatus. Recommended concentration of ab121240 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-FAM65A antibody (ab121240)
All lanes : Anti-FAM65A antibody (ab121240) at 1/250 dilution
Lane 1 : Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Plasma lysate
Lane 5 : Human liver lysate
Lane 6 : Huam tonsil lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM65A antibody (ab121240)
Immunohistochemical staining of paraffin embedded Human liver tissue, using 1/35 ab121240, shows strong nuclear and cytoplasmic positivity in hepatocytes.
References for Anti-FAM65A antibody (ab121240)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"