Anti-FAM69B antibody - N-terminal (ab170354)

Overview

  • Product name
    Anti-FAM69B antibody - N-terminal
    See all FAM69B primary antibodies
  • Description
    Rabbit polyclonal to FAM69B - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human FAM69B aa 114-144 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q5VUD6

  • Positive control
    • Mouse cerebellum lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 49 kDa.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse heart tissue labeling FAM69B with ab170354 at 1/50 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.

  • Anti-FAM69B antibody - N-terminal (ab170354) at 1/100 dilution + Mouse cerebellum tissue lysate at 35 µg

    Predicted band size: 49 kDa

References

ab170354 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA
Permeabilization
No
Specification
liver
Blocking step
Dako X0909 as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Sep 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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