Anti-PTPIP51 antibody (ab224081)
Key features and details
- Rabbit polyclonal to PTPIP51
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PTPIP51 antibody -
Description
Rabbit polyclonal to PTPIP51 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan -
Immunogen
Recombinant fragment corresponding to Human PTPIP51 aa 150-350.
Database link: Q96TC7 -
Positive control
- WB: Caco-2 cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human testis and skin tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 52 kDa.
|
|
IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 52 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Relevance
FAM82A2, or family with sequence similarity 82, member C, may participate in differentiation and apoptosis of keratinocytes. Overexpression of FAM82A2 induces apoptosis. -
Cellular localization
Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Nucleus. Note=In interphase localizes in the cytoplasm, and during mitosis localizes to the spindle microtubules and spindle poles. -
Database links
- Entrez Gene: 55177 Human
- Entrez Gene: 100172768 Orangutan
- Omim: 611873 Human
- SwissProt: Q96TC7 Human
- SwissProt: Q5R6Z1 Orangutan
-
Alternative names
- Cerebral protein 10 antibody
- FAM82C antibody
- FAM82C, formerly antibody
see all
Images
-
Anti-PTPIP51 antibody (ab224081) at 1/250 dilution + Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Predicted band size: 52 kDa -
Paraffin-embedded human testis tissue staining PTPIP51 with ab224081 at 1/500 dilution in immunohistochemical analysis. Strong cytoplasmic positibity in seminiferous duct cells.
-
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PTPIP51 (green) using ab224081 (4 µg/ml) in ICC/IF.
-
Paraffin-embedded human skeletal muscle tissue staining PTPIP51 with ab224081 at 1/500 dilution in immunohistochemical analysis. Weak cytoplasmic positivity in myocytes.
-
Paraffin-embedded human skin tissue staining PTPIP51 with ab224081 at 1/500 dilution in immunohistochemical analysis. Moderate to strong cytoplasmic positibity in keratinocytes.
-
Paraffin embedded human testis tissue stained for PTPIP51 with ab224081 (1/1000 dilution) in immunohistochemical analysis.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab224081 has been referenced in 1 publication.
- Rimessi A et al. Pharmacological modulation of mitochondrial calcium uniporter controls lung inflammation in cystic fibrosis. Sci Adv 6:eaax9093 (2020). PubMed: 32494695