Overview

  • Product name
  • Description
    Rabbit polyclonal to FAM96B
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 23-53 of Human FAM96B, conjugated to KLH.

  • Positive control
    • A375 cell line lysates; Human skin tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab103227 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD.
  • Sequence similarities
    Belongs to the MIP18 family.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 128 antibody
    • FAM96B antibody
    • Family with sequence similarity 96, member B antibody
    • HSPC118 antibody
    • Hypothetical protein LOC51647 antibody
    • MIP18 antibody
    • MIP18_HUMAN antibody
    • Mitotic spindle-associated MMXD complex subunit MIP18 antibody
    • MSS19-interacting protein of 18 kDa antibody
    • Protein FAM96B antibody
    see all

Images

  • Anti-FAM96B antibody (ab103227) at 1/100 dilution + A375 cell line lysates at 35 µg

    Predicted band size : 18 kDa
  • ab103227, at a 1/50 dilution, staining FAM96B in formalin fixed, paraffin embedded Human skin tissue by Immunohistochemistry, followed by peroxidase conjugated secondary antibody and DAB staining.

References

This product has been referenced in:
  • van Wietmarschen N  et al. The Mammalian Proteins MMS19, MIP18, and ANT2 Are Involved in Cytoplasmic Iron-Sulfur Cluster Protein Assembly. J Biol Chem 287:43351-8 (2012). WB . Read more (PubMed: 23150669) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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