Recombinant Anti-FAM98B antibody [EPR12872] (ab179833)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12872] to FAM98B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FAM98B antibody [EPR12872]
See all FAM98B primary antibodies -
Description
Rabbit monoclonal [EPR12872] to FAM98B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human FAM98B. The exact sequence is proprietary.
Database link: Q52LJ0 -
Positive control
- Raji, Caco-2 and Human fetal brain lysate. Human endometrial carcinoma, Human lung, Human pancreas tissue. Caco-2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12872 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
Target
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Relevance
The specific function of FAM98B is not yet known. -
Database links
- Entrez Gene: 283742 Human
- Entrez Gene: 68215 Mouse
- Entrez Gene: 499866 Rat
- SwissProt: Q52LJ0 Human
- SwissProt: Q80VD1 Mouse
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Alternative names
- family with sequence similarity 98, member B antibody
Images
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All lanes : Anti-FAM98B antibody [EPR12872] (ab179833) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : Human fetal brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 37 kDa -
Immunofluorescent analysis of Caco-2 cells labeling FAM98B with ab179833 at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling FAM98B with ab179833 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling FAM98B with ab179833 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling FAM98B with ab179833 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab179833 has been referenced in 2 publications.
- Zhou YT et al. Effect of functional variant rs11466313 on breast cancer susceptibility and TGFB1 promoter activity. Breast Cancer Res Treat 184:237-248 (2020). PubMed: 32757134
- Pazo A et al. hCLE/RTRAF-HSPC117-DDX1-FAM98B: A New Cap-Binding Complex That Activates mRNA Translation. Front Physiol 10:92 (2019). PubMed: 30833903