Customer reviews and FAQs

Overview

  • Product name
    Anti-FAN1 antibody
    See all FAN1 primary antibodies
  • Description
    Mouse polyclonal to FAN1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human MTMR15 protein (AAH47882, 1 a.a. ~ 533 a.a)

  • Positive control
    • Human spleen lysate. MTMR15 transfected 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).

Target

  • Function
    Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.
  • Sequence similarities
    Belongs to the FAN1 family.
    Contains 1 UBZ-type zinc finger.
    Contains 1 VRR-NUC domain.
  • Domain
    The UBZ-type zinc finger specifically binds monoubiquitinated FANCD2.
  • Cellular localization
    Nucleus. Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2.
  • Information by UniProt
  • Database links
    • Alternative names
      • 6030441H18Rik antibody
      • Coiled coil domain containing protein MTMR15 antibody
      • DKFZp451H236 antibody
      • DKFZp686K16147 antibody
      • FAN1 antibody
      • FAN1_HUMAN antibody
      • FANCD2 and FANCI associated nuclease 1 antibody
      • FANCD2/FANCI associated nuclease 1 antibody
      • FANCD2/FANCI-associated nuclease 1 antibody
      • Fanconi associated nuclease 1 antibody
      • Fanconi-associated nuclease 1 antibody
      • hFAN1 antibody
      • KIAA1018 antibody
      • KMIN antibody
      • MTMR 15 antibody
      • MTMR15 antibody
      • Myotubularin related protein 15 antibody
      • Myotubularin-related protein 15 antibody
      see all

    Anti-FAN1 antibody images

    • Western blot - MTMR15 antibody (ab68572)
      Western blot - MTMR15 antibody (ab68572)
      Anti-FAN1 antibody (ab68572) at 1/500 dilution + Human spleen lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 114 kDa
      Observed band size : 114 kDa
    • Western blot - MTMR15 antibody (ab68572)
      Western blot - MTMR15 antibody (ab68572)
      All lanes : Anti-FAN1 antibody (ab68572) at 1/500 dilution

      Lane 1 : MTMR15 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 114 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)

    References for Anti-FAN1 antibody (ab68572)

    ab68572 has not yet been referenced specifically in any publications.

    Publishing research using ab68572? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

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    Western blot abreview for Anti-FAN1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cell line)
    Loading amount
    40 µg
    Specification
    HeLa cell line
    Gel Running Conditions
    Reduced Denaturing (6%)
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
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    Verified customer

    Submitted Oct 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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