- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-FAN1 antibody
See all FAN1 primary antibodies -
DescriptionMouse polyclonal to FAN1
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Host speciesMouse
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Full length human MTMR15 protein (AAH47882, 1 a.a. ~ 533 a.a)
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Positive control
- Human spleen lysate. MTMR15 transfected 293T cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab68572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa). |
Target
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FunctionNuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.
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Sequence similaritiesBelongs to the FAN1 family.
Contains 1 UBZ-type zinc finger.
Contains 1 VRR-NUC domain. -
DomainThe UBZ-type zinc finger specifically binds monoubiquitinated FANCD2.
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Cellular localizationNucleus. Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2.
- Information by UniProt
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Database links
- Entrez Gene: 22909 Human
- Omim: 613534 Human
- SwissProt: Q9Y2M0 Human
- Unigene: 584863 Human
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Alternative names
- 6030441H18Rik antibody
- Coiled coil domain containing protein MTMR15 antibody
- DKFZp451H236 antibody
see all
Images
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Western blot - MTMR15 antibody (ab68572)Anti-FAN1 antibody (ab68572) at 1/500 dilution + Human spleen lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa -
Western blot - MTMR15 antibody (ab68572)All lanes : Anti-FAN1 antibody (ab68572) at 1/500 dilution
Lane 1 : MTMR15 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 114 kDa
Observed band size: 70 kDa (why is the actual band size different from the predicted?)
Protocols
References
ab68572 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"