Overview

  • Product nameAnti-FAN1 antibody
    See all FAN1 primary antibodies
  • Description
    Mouse polyclonal to FAN1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human MTMR15 protein (AAH47882, 1 a.a. ~ 533 a.a)

  • Positive control
    • Human spleen lysate. MTMR15 transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).

Target

  • FunctionNuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.
  • Sequence similaritiesBelongs to the FAN1 family.
    Contains 1 UBZ-type zinc finger.
    Contains 1 VRR-NUC domain.
  • DomainThe UBZ-type zinc finger specifically binds monoubiquitinated FANCD2.
  • Cellular localizationNucleus. Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6030441H18Rik antibody
    • Coiled coil domain containing protein MTMR15 antibody
    • DKFZp451H236 antibody
    • DKFZp686K16147 antibody
    • FAN1 antibody
    • FAN1_HUMAN antibody
    • FANCD2/FANCI associated nuclease 1 antibody
    • FANCD2/FANCI-associated nuclease 1 antibody
    • Fanconi associated nuclease 1 antibody
    • Fanconi-associated nuclease 1 antibody
    • KIAA1018 antibody
    • MTMR 15 antibody
    • MTMR15 antibody
    • Myotubularin related protein 15 antibody
    • Myotubularin-related protein 15 antibody
    see all

Anti-FAN1 antibody images

  • Anti-FAN1 antibody (ab68572) at 1/500 dilution + Human spleen lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 114 kDa
    Observed band size : 114 kDa
  • All lanes : Anti-FAN1 antibody (ab68572) at 1/500 dilution

    Lane 1 : MTMR15 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 114 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-FAN1 antibody (ab68572)

ab68572 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cell line)
Loading amount 40 µg
Specification HeLa cell line
Gel Running Conditions Reduced Denaturing (6%)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"