Recombinant
RabMAb

Anti-FAN1 antibody [EPR14350] (ab185554)

Overview

  • Product name
    Anti-FAN1 antibody [EPR14350]
    See all FAN1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14350] to FAN1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human FAN1 aa 50-250. The exact sequence is proprietary.
    Database link: Q9Y2M0

  • Positive control
    • PC3 and 293 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14350
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab185554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 114 kDa).

Target

  • Function
    Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.
  • Sequence similarities
    Belongs to the FAN1 family.
    Contains 1 UBZ-type zinc finger.
    Contains 1 VRR-NUC domain.
  • Domain
    The UBZ-type zinc finger specifically binds monoubiquitinated FANCD2.
  • Cellular localization
    Nucleus. Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6030441H18Rik antibody
    • Coiled coil domain containing protein MTMR15 antibody
    • DKFZp451H236 antibody
    • DKFZp686K16147 antibody
    • FAN1 antibody
    • FAN1_HUMAN antibody
    • FANCD2 and FANCI associated nuclease 1 antibody
    • FANCD2/FANCI associated nuclease 1 antibody
    • FANCD2/FANCI-associated nuclease 1 antibody
    • Fanconi associated nuclease 1 antibody
    • Fanconi-associated nuclease 1 antibody
    • hFAN1 antibody
    • KIAA1018 antibody
    • KMIN antibody
    • MTMR 15 antibody
    • MTMR15 antibody
    • Myotubularin related protein 15 antibody
    • Myotubularin-related protein 15 antibody
    see all

Images

  • All lanes : Anti-FAN1 antibody [EPR14350] (ab185554) at 1/5000 dilution

    Lane 1 : PC3 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 114 kDa

    Blocking buffer: 5% NFDM/TBST

References

ab185554 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab185554.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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