Overview

  • Product name
  • Description
    Rabbit polyclonal to FANCA
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 190 of FANCA (O15360).

  • Positive control
    • A431, HeLa, 293T or H1299 cell lines.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 163 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
  • Involvement in disease
    Defects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.
  • Cellular localization
    Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • FA 1 antibody
    • FA antibody
    • FA H antibody
    • FA1 antibody
    • FAA antibody
    • FACA antibody
    • FAH antibody
    • Fanca antibody
    • FANCA_HUMAN antibody
    • FANCH antibody
    • Fanconi anemia complementation group A antibody
    • Fanconi anemia complementation group H antibody
    • Fanconi anemia group A protein antibody
    • Fanconi anemia type 1 antibody
    • MGC75158 antibody
    • Protein FACA antibody
    see all

Images

  • All lanes : Anti-FANCA antibody (ab97578) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 163 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97578 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.
  • Immunohistochemistry analysis of paraffin-embedded OV90 xenograft, using ab97578 at 1/500 dilution.

References

This product has been referenced in:
  • Jamsai D  et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS One 8:e56955 (2013). WB ; Mouse . Read more (PubMed: 23451117) »

See 1 Publication for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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