Overview

  • Product nameAnti-FANCD2 antibody
    See all FANCD2 primary antibodies
  • Description
    Rabbit polyclonal to FANCD2
  • SpecificityThis antibody is specific for human FANC D2.
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    This antibody was made to an N-terminal fusion protein fragment (human).

  • Positive control
    • HeLa whole cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: Tris glycine, 150mM Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/500.
WB 1/10000 - 1/20000. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).
IP Use at an assay dependent dilution.

Target

  • FunctionRequired for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
  • Tissue specificityHighly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
  • Involvement in diseaseDefects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 (FANCD2) [MIM:227646]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • Developmental stageHighly expressed in fetal oocytes, and in hematopoietic cells of the fetal liver and bone marrow (at protein level).
  • DomainThe C-terminal 24 residues of isoform 2 are required for its function.
  • Post-translational
    modifications
    Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex, RPA1 and ATR, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1.
    Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.
  • Cellular localizationNucleus. Concentrates in nuclear foci during S phase and upon genotoxic stress.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp762A223 antibody
    • FA 4 antibody
    • FA D2 antibody
    • FA4 antibody
    • FAC D2 antibody
    • FACD 2 antibody
    • FACD antibody
    • FACD2 antibody
    • FACD2_HUMAN antibody
    • FAD antibody
    • FAD2 antibody
    • FANC D2 antibody
    • FANCD 2 antibody
    • FANCD antibody
    • FANCD2 antibody
    • FANCONI ANEMIA COMPLEMENTATION GROUP D antibody
    • Fanconi anemia complementation group D2 antibody
    • Fanconi anemia group D2 protein antibody
    • FANCONI PANCYTOPENIA TYPE 4 antibody
    • FLJ23826 antibody
    • OTTHUMP00000158853 antibody
    • OTTHUMP00000207925 antibody
    • Protein FACD2 antibody
    • Type 4 Fanconi pancytopenia antibody
    see all

Anti-FANCD2 antibody images

  • All lanes : Anti-FANCD2 antibody (ab2187) at 1 µg/ml

    Lane 1 : A431 whole cell lysate (ab7909)
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : HEK293 whole cell lysate (ab7902)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 166 kDa
    Observed band size : 165 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-FANCD2 antibody (ab2187) at 1/1500 dilution

    Lane 1 : Wild type human breast - whole cell lysate (positive control)
    Lane 2 : Transient D2 Knockdown 24 hours after transfection - whole cell lysate
    Lane 3 : Transient D2 Knockdown 48 hours after transfection - whole cell lysate
    Lane 4 : Transient D2 Knockdown 120 hours after transfection - whole cell lysate

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgA - alpha chain (ab2758) at 1/1500 dilution

    Predicted band size : 166 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Mr Richard Allum

    See Abreview

  • ab2187 at 1:500 staining FANCD2 in SiHa cells by ICC/IF. The proliferating SiHa cells were exposed to 10 Gy of IR and then double immunofluorescence staining was performed after 8 hours.

  • ab2187 at 2.5µg/ml staining FANCD2 in human prostate glandular tissue section by Immunohistochemistry (Fromlain/ PFA fixed paraffin-embedded tissue sections).

References for Anti-FANCD2 antibody (ab2187)

This product has been referenced in:
  • Luebben SW  et al. A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression. Nucleic Acids Res 42:5605-15 (2014). WB, ICC, IHC ; Mouse . Read more (PubMed: 24589582) »
  • Yeo JE  et al. CtIP mediates replication fork recovery in a FANCD2-regulated manner. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24556218) »

See all 27 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (3T3MEF)
Gel Running Conditions Non-reduced Denaturing (5% SDSpage)
Loading amount 50000 cells
Specification 3T3MEF
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 22 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample Human Cell (U2OS cells)
Specification U2OS cells
Permeabilization Yes - 0.2% triton
Fixative Paraformaldehyde
Username

Dr. Craig MacKay

Verified customer

Submitted Mar 21 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Human Cell lysate - whole cell (HeLa cell lysate)
Specification HeLa cell lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing
Sample Human Cell lysate - whole cell (HEK 293)
Specification HEK 293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. christophe lachaud

Verified customer

Submitted Aug 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (U2OS cells)
Specification U2OS cells
Permeabilization No
Fixative Methanol
Username

Abcam user community

Verified customer

Submitted Jul 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (human fibroblasts (PD20))
Specification human fibroblasts (PD20)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 24 2013

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cell line)
Total protein in input 40 µg
Specification HeLa cell line
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Nov 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cell line)
Specification HeLa cell line
Fixative Formaldehyde
Permeabilization Yes - 0.5% Tritón X+PBS1X
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 21 2012

Thank you for contacting Abcam earlier today.

As we discussed, I am sending you ab31577as a free of charge replacement for ab2187, as you were not seeing the correct signal in western blot. Your new order number is ********. Also, below is th...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Vascular Smooth Muscle Cell)
Loading amount 20 µg
Specification Vascular Smooth Muscle Cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Andrew Cobb

Verified customer

Submitted Apr 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"