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This antibody was made to an N-terminal fusion protein fragment (human).
Our Abpromise guarantee covers the use of ab2187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/500.|
|WB||1/10000 - 1/20000. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).|
|IP||Use at an assay dependent dilution.|
This image is courtesy of an Abreview submitted by Mr Richard Allum
ab2187 at 1:500 staining FANCD2 in SiHa cells by ICC/IF. The proliferating SiHa cells were exposed to 10 Gy of IR and then double immunofluorescence staining was performed after 8 hours.
ab2187 at 2.5µg/ml staining FANCD2 in human prostate glandular tissue section by Immunohistochemistry (Fromlain/ PFA fixed paraffin-embedded tissue sections).