Overview

  • Product name
  • Description
    Goat polyclonal to FANCE
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    C-EHKSLESLADGGS

    , corresponding to internal sequence amino acids 235-247 of Human FANCE (NP_068741.1).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab105023 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 59 kDa. Preliminary experiments gave an approx 70kDa band in Human Adipose and Uterus lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.7kDa according to NP_068741.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
IHC-P Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    As part of the Fanconi anemia (FA) complex functions in DNA cross-links repair. Required for the nuclear accumulation of FANCC and provides a critical bridge between the FA complex and FANCD2.
  • Involvement in disease
    Fanconi anemia complementation group E
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation by CHEK1 at Thr-346 and Ser-374 regulates its function in DNA cross-links repair.
    Ubiquitinated. Phosphorylation by CHEK1 induces polyubiquitination and degradation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810451D06Rik antibody
    • AI415634 antibody
    • AW209126 antibody
    • FACE antibody
    • FAE antibody
    • FANCE antibody
    • FANCE_HUMAN antibody
    • Fanconi anemia complementation group E antibody
    • Fanconi anemia group E protein antibody
    • OTTHUMP00000016254 antibody
    • Protein FACE antibody
    • RGD1561045 antibody
    see all

Images

  • ab105023 (3.8µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

ab105023 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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