Overview

  • Product nameAnti-FANCE antibody
    See all FANCE primary antibodies
  • Description
    Rabbit polyclonal to FANCE
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (SPQAPDPEEE ENRDSQQPGK RRKDSEEEAA SPEGKRVPKR LRCWEEEEDH) of human FANCE (NP_068741).

  • Positive control
    • HepG2 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab83851 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab83851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAs part of the Fanconi anemia (FA) complex functions in DNA cross-links repair. Required for the nuclear accumulation of FANCC and provides a critical bridge between the FA complex and FANCD2.
  • Involvement in diseaseFanconi anemia complementation group E
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation by CHEK1 at Thr-346 and Ser-374 regulates its function in DNA cross-links repair.
    Ubiquitinated. Phosphorylation by CHEK1 induces polyubiquitination and degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810451D06Rik antibody
    • AI415634 antibody
    • AW209126 antibody
    • FACE antibody
    • FAE antibody
    • FANCE antibody
    • FANCE_HUMAN antibody
    • Fanconi anemia complementation group E antibody
    • Fanconi anemia group E protein antibody
    • OTTHUMP00000016254 antibody
    • Protein FACE antibody
    • RGD1561045 antibody
    see all

Anti-FANCE antibody images

  • Anti-FANCE antibody (ab83851) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 59 kDa

References for Anti-FANCE antibody (ab83851)

ab83851 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 2% TritonX100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: RT°C
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Submitted Mar 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"