The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
FunctionDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
Involvement in diseaseDefects in FANCF are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
Lane 1 : Anti-FANCF antibody (ab109585) at 0.5 µg/ml Lane 2 : Anti-FANCF antibody (ab109585) at 1 mg/ml Lane 3 : anti-MYC Tag at 1/1000 dilution
Lane 1 : HEK293 lysate overexpressing Human FANCF with C-terminal MYC and DYKDDDDK tags Lane 2 : HEK293 lysate, mock-transfected Lane 3 : HEK293 lysate overexpressing Human FANCF with C-terminal MYC and DYKDDDDK tags
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Predicted band size : 42 kDa
Exposure time : 1 hour
References for Anti-FANCF antibody (ab109585)
has not yet been referenced specifically in any publications.
Publishing research using ab109585? Please let us know so that we can cite the reference in this datasheet.
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