Overview

  • Product name
  • Description
    Goat polyclonal to FANCF
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-RQVLGLSAGLSSV

    , corresponding to C terminal amino acids 362-374 of Human FANCF (NP_073562).

  • Positive control
    • FANCF transfected HEK293 lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.

Target

  • Function
    DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
  • Involvement in disease
    Defects in FANCF are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A730016A17 antibody
    • FACF antibody
    • FAF antibody
    • FANCF antibody
    • FANCF_HUMAN antibody
    • Fanconi anemia complementation group F antibody
    • Fanconi anemia group F protein antibody
    • MGC126856 antibody
    • Protein FACF antibody
    • RGD1561456 antibody
    see all

Images

  • Lane 1 : Anti-FANCF antibody (ab109585) at 0.5 µg/ml
    Lane 2 : Anti-FANCF antibody (ab109585) at 1 mg/ml
    Lane 3 : anti-MYC Tag at 1/1000 dilution

    Lane 1 : HEK293 lysate overexpressing Human FANCF with C-terminal MYC and DYKDDDDK tags
    Lane 2 : HEK293 lysate, mock-transfected
    Lane 3 : HEK293 lysate overexpressing Human FANCF with C-terminal MYC and DYKDDDDK tags

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 42 kDa


    Exposure time : 1 hour

References

ab109585 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109585.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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