Overview

  • Product name
  • Description
    Goat polyclonal to FANCG
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Pig, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide:

    LEEFRTSLPKSCDL

    , corresponding to C terminal amino acids 609-622 of Human FANCG

  • Positive control
    • HeLa cell lysate, Jurkat cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.
    • Tissue specificity
      Highly expressed in testis and thymus. Found in lymphoblasts.
    • Involvement in disease
      Defects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair.
    • Sequence similarities
      Contains 4 TPR repeats.
    • Cellular localization
      Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA repair protein XRCC9 antibody
      • FAG antibody
      • FANCG antibody
      • FANCG_HUMAN antibody
      • Fanconi anaemia complementation group G antibody
      • Fanconi anemia group G protein antibody
      • Protein FACG antibody
      • X ray repair, complementing defective, in Chinese hamster cells 9 antibody
      • X-ray repair, complementing defective, in Chinese hamster, 9 antibody
      • XRCC9 antibody
      see all

    Images

    • Anti-FANCG antibody (ab77193) at 0.5 µg/ml + Jurkat nuclear lysate in RIPA buffer at 35 µg

      Predicted band size : 69 kDa
    • Anti-FANCG antibody (ab77193) at 0.5 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 69 kDa
      Observed band size : 69 kDa


      Exposure time : 1 hour

    References

    ab77193 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77193.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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