Overview

  • Product nameAnti-FANCI antibody
    See all FANCI primary antibodies
  • Description
    Rabbit polyclonal to FANCI
  • Tested applicationsSuitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab15344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IP
WB
  • Application notesICC/IF: Use at an assay dependent dilution (PMID 19465922).
    IP: Use at an assay dependent dilution.
    WB: 1/2500 - 1/10000. Detects a band of approximately 145 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
    • Involvement in diseaseDefects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    • DomainThe C-terminal 30 residues are probably required for function in DNA repair.
    • Post-translational
      modifications
      Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
      Phosphorylated in response to DNA damage by ATM and/or ATR.
    • Cellular localizationNucleus. Concentrates in nuclear foci upon genotoxic stress.
    • Information by UniProt
    • Database links
    • Alternative names
      • FANCI antibody
      • FANCI gene antibody
      • FANCI_HUMAN antibody
      • Fanconi anemia group I protein antibody
      • Fanconi anemia, complementation group I antibody
      • FLJ10719 antibody
      • FLJ14658 antibody
      • KIAA1794 antibody
      • Protein FACI antibody
      • Protein FANCI antibody
      see all

    Anti-FANCI antibody images

    • All lanes : Anti-FANCI antibody (ab15344) at 1/5000 dilution

      Lane 1 : HeLa cell nuclear extract 5 µg.
      Lane 2 : HeLa cell nuclear extract 20 µg.


      Observed band size : 145 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 160 kDa,230 kDa,70 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 15 minutes

      Detection: chemiluminescence.
    • ab15344 (1/400) staining FANCI in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

      See Abreview

    References for Anti-FANCI antibody (ab15344)

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12 Bis tris)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 31 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 25 2014

    Thank you for submitting an Abreview of ab15344. As you may have noticed, your review has now been published on our website.

    Since you obtained poor results using the antibody in a tested species and application, we would like to follow up o...

    Read More
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HeLa cell line)
    Total protein in input 40 µg
    Specification HeLa cell line
    Immuno-precipitation step Protein G
    Username

    Abcam user community

    Verified customer

    Submitted Sep 28 2012

    Thank you for your interest in our antibodies. We do not provide samples of our antibodies as under our Abpromise we guarantee our antibodies to work as stated on the data sheet. In the case of ab15344, ab74332 and ab15346as they are only guaranteed to...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton-X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Nov 14 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Formaldehyde
    Permeabilization Yes - 0.5% Triton X-100
    Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 28 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 40 µg
    Specification HeLa
    Treatment 0.5µg/mL MMC for 24h
    Gel Running Conditions Reduced Denaturing (6%)
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"