Overview

  • Product name
  • Description
    Rabbit polyclonal to FANCI
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1025 and 1075 of human KIAA1794 (NP_060663.2)

  • Positive control
    • Whole cell lysates from HeLa and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
  • Involvement in disease
    Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • Domain
    The C-terminal 30 residues are probably required for function in DNA repair.
  • Post-translational
    modifications
    Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
    Phosphorylated in response to DNA damage by ATM and/or ATR.
  • Cellular localization
    Nucleus. Concentrates in nuclear foci upon genotoxic stress.
  • Information by UniProt
  • Database links
  • Alternative names
    • FANCI antibody
    • FANCI gene antibody
    • FANCI_HUMAN antibody
    • Fanconi anemia group I protein antibody
    • Fanconi anemia, complementation group I antibody
    • FLJ10719 antibody
    • FLJ14658 antibody
    • KIAA1794 antibody
    • Protein FACI antibody
    • Protein FANCI antibody
    see all

Images

  • All lanes : Anti-FANCI antibody (ab74332) at 0.04 µg/ml

    Lane 1 : Whole HeLa cell lysate at 50 µg
    Lane 2 : Whole HeLa cell lysate at 15 µg
    Lane 3 : Whole HeLa cell lysate at 5 µg
    Lane 4 : Whole 293T cell lysate at 50 µg


    Predicted band size : 149 kDa
    Additional bands at : 100 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human KIAA1794 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab74332 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents IgG control IP.

References

This product has been referenced in:
  • Jaber S  et al. p53 downregulates the Fanconi anaemia DNA repair pathway. Nat Commun 7:11091 (2016). WB . Read more (PubMed: 27033104) »
  • Luebben SW  et al. A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression. Nucleic Acids Res 42:5605-15 (2014). ICC/IF ; Mouse . Read more (PubMed: 24589582) »

See all 4 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab74332.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up