• Product nameAnti-FANCI antibody
    See all FANCI primary antibodies
  • Description
    Rabbit polyclonal to FANCI
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1025 and 1075 of human KIAA1794 (NP_060663.2)

  • Positive control
    • Whole cell lysates from HeLa and 293T cells.


Associated products


Our Abpromise guarantee covers the use of ab74332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 149 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
    • Involvement in diseaseDefects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
    • DomainThe C-terminal 30 residues are probably required for function in DNA repair.
    • Post-translational
      Monoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
      Phosphorylated in response to DNA damage by ATM and/or ATR.
    • Cellular localizationNucleus. Concentrates in nuclear foci upon genotoxic stress.
    • Information by UniProt
    • Database links
    • Alternative names
      • FANCI antibody
      • FANCI gene antibody
      • FANCI_HUMAN antibody
      • Fanconi anemia group I protein antibody
      • Fanconi anemia, complementation group I antibody
      • FLJ10719 antibody
      • FLJ14658 antibody
      • KIAA1794 antibody
      • Protein FACI antibody
      • Protein FANCI antibody
      see all

    Anti-FANCI antibody images

    • All lanes : Anti-FANCI antibody (ab74332) at 0.04 µg/ml

      Lane 1 : Whole HeLa cell lysate at 50 µg
      Lane 2 : Whole HeLa cell lysate at 15 µg
      Lane 3 : Whole HeLa cell lysate at 5 µg
      Lane 4 : Whole 293T cell lysate at 50 µg

      Predicted band size : 149 kDa
      Additional bands at : 100 kDa,65 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human KIAA1794 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab74332 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents IgG control IP.

    References for Anti-FANCI antibody (ab74332)

    This product has been referenced in:
    • Luebben SW  et al. A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression. Nucleic Acids Res 42:5605-15 (2014). WB, ICC, IHC ; Mouse . Read more (PubMed: 24589582) »
    • Jamsai D  et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS One 8:e56955 (2013). WB ; Mouse . Read more (PubMed: 23451117) »

    See all 3 Publications for this product

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