The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites.
Involvement in diseaseDefects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
DomainThe C-terminal 30 residues are probably required for function in DNA repair.
Post-translational modificationsMonoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Phosphorylated in response to DNA damage by ATM and/or ATR.
Cellular localizationNucleus. Concentrates in nuclear foci upon genotoxic stress.
All lanes : Anti-FANCI antibody (ab74332) at 0.04 µg/ml
Lane 1 : Whole HeLa cell lysate at 50 µg Lane 2 : Whole HeLa cell lysate at 15 µg Lane 3 : Whole HeLa cell lysate at 5 µg Lane 4 : Whole 293T cell lysate at 50 µg
Predicted band size : 149 kDa Additional bands at : 100 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - KIAA1794 antibody (ab74332)
Detection of Human KIAA1794 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab74332 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents IgG control IP.
References for Anti-FANCI antibody (ab74332)
This product has been referenced in:
Luebben SW et al. A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression. Nucleic Acids Res42:5605-15 (2014).
WB, ICC, IHC
Read more (PubMed: 24589582) »
Jamsai D et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS One8:e56955 (2013).
Read more (PubMed: 23451117) »